The result obtained from the test shows excellent zone of inhibition against Escherichia coli and Staphylococcus aureus. The physical and comfort properties of the treated fabric was slightly affected when compared with untreated fabric. The treated fabric shows excellent wound healing property using in vitro self-wound healing scratch assay test. Thus, Rosmarinus officinalis and Psidium guajava extract treated fabric may be preferred for making wound dressing, compression bandages, head cover, face mask, patient dress material, and surgical gowns for health care and hygienic applications.